"Генетика", 2024 г., № 10

Статьи, опубликованные только в Russian J. of Genetics, № 10 – 2024 г.

TaATG8 Is Involved in the Response to Abiotic Stresses and Powdery Mildew in Arabidopsis

L. J. Tian¹, G. Liu^1,2, R. N. Wang^1,2, F. J. Wei^1,2, F. F. Zhang¹, C. Y. Hou^1,2, D. M. Wang^1,2

¹ Plant Stress Laboratory, College of Life Science, Hebei Agricultural University, 071001, Baoding, P.R. China
² StateKey Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, 071001, Baoding, P.R. China
Correspondence to G. Liu or D. M. Wang

ATG8 is a key gene in the process of autophagy. The expression of ATG8 affects many intracellular progresses. Here we report that the amino acid sequence of AtATG8 and TaATG8 protein share a high similarity of 84.03%, we found that TaATG8 involved in the abiotic stresses, such as drought, salt stress and nutrient deficiency. Moreover, TaATG8 plays an important role in the process of Arabidopsis defending against biotic stress caused by the infection of powdery mildew through HR. Hydrogen peroxide acts as an important metabolic product and signaling molecule in the process of TaATG8 resisting to powdery mildew.

DOI: 10.1134/S1022795424700893
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Mining of Novel Simple Sequence Repeat (SSR) Markers Derived from Chicken (Gallus gallus) Genome Assembly

Z. Hajibarat¹, A. Saidi¹, Mehrshad Zeinalabedini², M. Mardi², M. R. Ghaffari²

¹ Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
² Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, Iran
Correspondence to A. Saidi or Mehrshad Zeinalabedini

Producing food for animals and providing meat and eggs to humans can be done economically through chicken. The creation of novel SSR markers for chicken will be an invaluable tool for evaluating genetic diversity, mapping quantitative trait loci (QTLs), and understanding population structure, and marker-assisted animal breeding applications. On the other hand, the identification of microsatellites is expensive and labor-intensive. The GMATA software was used in the current in silico approach to generate microsatellites with chromosome-anchored SSR markers from the chicken genome assembly. The genome assembly of chicken resulted in the development of 146 054 SSR markers, and marker loci that corresponded to protein coding sequences were determined. Dinucleotide motifs were prevailing (57.92%) in Ross genome. Functional analysis was used to infer the existence of SSR repeats in biologically important classes of proteins, such as kinases, transcription factors, and G protein receptors. These outcome indicated that variability of SSR repeat copies can endow the cell and whole genome and can be realized in KEGG pathways namely involvement of neuroactive ligand-receptor interaction, MAPK signaling pathway, melanogenesis, WNT signaling pathway, and adherens junction. For the first time, the present study developed a whole-genome SSR panel for chicken as part of its research. The considerable quantity of new SSR markers presented in this study serves as a significant asset for molecular genetic studies involving QTL mapping, evaluation of genetic variation, and the use of markers in animal breeding.

DOI: 10.1134/S1022795424700923
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LncRNA linc00641 Inhibits the Proliferation and Differentiation of Chondrocytes and Aggravates Joint Injury by Targeting miR-320a

Sh. Y. Han¹, H. Liu², Y. Wang², Sh. Y. Wang², B. Yang², D. Sun¹

¹ Department of Orthopedics, Peking University Third Hospital Qinhuangdao Hospital, 066000, Qinhuangdao, China
² Department of Nursing, Peking University Third Hospital Qinhuangdao Hospital, 066000, Qinhuangdao, China
Correspondence to Sh. Y. Han

The purpose of this study was to investigate the expression of long non-coding RNA linc00641 in OA and its biological function in osteoarthritis (OA). The expression level of linc00641 in serum and cartilage tissue of OA subjects was detected by qRT-PCR. LPS-induced C28/I2 cells were used to construct cartilage injury models in vitro. The effects of linc00641 on the viability, apoptosis, inflammation, proliferation, and differentiation of chondrocyte models were evaluated. The target gene of linc00641 was predicted to be miR-320 through the database, and the relationship between linc00641 and miR-320a was verified using luciferase reporter gene assay. The effect of miR-320a on chondrocyte model was further evaluated. Linc00641 was significantly upregulated in serum and cartilage tissue of patients with knee OA. Functionally, downregulation of linc00641 can improve the inhibition of proliferation and differentiation of chondrocytes induced by LPS, as well as the promotion of apoptosis and inflammation. In addition, linc00641 can target and regulate the expression of miR-320a. Reduced expression of miR-320a reversed the positive effects of linc00641 inhibition on C28/I2 cell function. Linc00641 may inhibit proliferation and differentiation of C28/I2 cells through targeted inhibition of miR-320a, thus increasing the injury of cartilage tissue during OA.

DOI: 10.1134/S1022795424700960
К статье на сайте SpringerLink

Evidence for a Causal Relationship between Heart Failure and Cerebral Infarction: A Bidirectional Two-Sample Mendelian Randomization Study

Zh. Cai¹, Yh. Zhang¹, X. Li^2,3, Wq. Sun¹

¹ Department of Cardiology, The Third People’s Hospital of Datong, 037000, Datong, Shanxi, China
² Clinical Research Center, The Third People’s Hospital of Datong, 037000, Datong, Shanxi, China
³ The Third Clinical College, Shanxi University of Chinese Medicine, 030024, Taiyuan, Shanxi, China
Correspondence to Wq. Sun

Previous studies have shown a potential correlation between heart failure (HF) and cerebral infarction (CI), but its genetic basis is still unclear. This study aim to explore the genetic correlation and potential causal relationships between HF and CI. To analyse the risk relationship between heart failure and cerebral infarction by two-sample bidirectional Mendelian randomization (MR) method using human genome-wide association data analysis (GWAS). Pooled data extracted from GWAS were analyzed to explore the correlation between HF and CI risk in a European population using a two-sample bidirectional MR method. Single nucleotide polymorphisms (SNPs) strongly associated with HF but not with CI were selected as genetic instrumental variables and analyzed using inverse variance weighted (IVW) analysis, weighted median analysis, simple mode, weighted mode and MR-Egger regression to investigate the causal relationship between HF and CI risk. Simultaneously, the stability and accuracy of the results were evaluated through horizontal pleiotropy, heterogeneity testing and exclusion sensitivity testing. IVW analysis showed a causal relationship between the two diseases, with HF being a risk factor for CI (OR = 1.0062, 95% CI 1.0000–1.0125, P = 0.0000), and there was no horizontal pleiotropy (Egger interval = –3.21E-04, P = 0.179) and heterogeneity (P = 0.5613, P = 0.4618). Other statistical methods, such as sensitivity analysis, did not find genetic pleiotropy biasing the results. In contrast, reverse MR results showed no significant causal relationship between CI and HF (OR = 1.89, 95% CI = 0.02–2.84, P = 0.788), and CI was not a risk factor for the development of HF. In a European population, HF may have a unidirectional causal association with CI, but no direct effect independent of HF on CI.

DOI: 10.1134/S1022795424700972
К статье на сайте SpringerLink