"Генетика", 2024 г., № 4

Статьи, опубликованные только в Russian J. of Genetics, № 5 – 2024 г.

Overexpression of LbPT7 Promotes Phosphorus Uptake by Plants and Affects Phosphorus Uptake by Arbuscular Mycorrhizas under High Phosphorus Condition

Y. Li¹, Y. Zhou¹, X. Han¹, M. Tang^1,2, H. Zhang¹

¹ College of Forestry, Northwest A&F University, 712100, Yangling, Shaanxi, China
² State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, College of Forestry and Landscape Architecture, South China Agricultural University, 510642, Guangzhou, China
Correspondence to H. Zhang

Previous studies have proved that arbuscular mycorrhizal fungi infection and phosphate transporters are particularly important for phosphorus uptake by plants. Early laboratory studies found that LbPT7 played a role in Pi uptake and it was affected by arbuscular mycorrhizal fungi. In order to verify the phosphorus uptake function of LbPT7 gene and the effect of interaction with arbuscular mycorrhiza on plants, we constructed LbPT7 overexpression vector with Nicotiana tabacum and obtained T1 generation overexpression positive plants as test material. Under the condition of pot experiment, we designed three factors: Arbuscular Mycorrhizal Fungi treatment, genotype treatment, phosphorus concentration treatment. After 4 weeks of treatment, we measured the biomass and phosphorus content of tobacco, and determined the colonization. The results showed that overexpression of LbPT7 can promote plant phosphorus uptake and inoculation of AM fungi further promote plant phosphorus uptake in phosphorus deficiency condition, but this effect of them can’t superpose in phosphorus adequate conditions. Overexpression of LbPT7 promoted the absorption of phosphorus, but impeded the utilization of phosphorus of N. tabacum in phosphorus deficiency condition. Overexpression of LbPT7 had little effect on the colonization of AM fungi in N. tabacum.

DOI: 10.1134/S1022795424700108
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The Controversial of Phylogenetic Status in an Unknown Orchidaceae Genus

WG. Li^2,3, JR. Liu², H. Sun^2,3, JY. Liu², K. Sun^2,3, L. Wang¹

¹ Department of Pharmacy, Anhui College of Traditional Chinese Medicine, 241000, Wuhu, Anhui, China
² College of Life Sciences, Anhui Normal University, 241000, Wuhu, Anhui, China
³ Anhui Provincial Key Laboratory of Conservation and Exploitation of Biological Resources, 241000, Wuhu, Anhui, China
Correspondence to K. Sun or L. Wang

Orchidaceae are one of the two largest families of flowering plants, new orchid genera are being described at a rate of about 13 per year. Bletilla sinensis is one of five species in the Bletilla. It has high medicinal value and can promote blood circulation and anti-inflammation. However, its phylogenetic status is controversial. In this study, the chloroplast genes of B. sinensis were sequenced and assembled, and download 73 complete chloroplast genes of orchid plants from NCBI to construct a molecular evolutionary tree, which provided new clues for the classification of B. sinensis. The results showed that the length of chloroplast genes was 156,941 bp, the GC content was 37.12%, and it contained 139 genes. The results of the molecular evolutionary tree show that the genus Bletilla is a single branch and has an independent evolutionary status. However, our sample is not in the same branch as the Bletilla, indicating that this sample may have an independent and effective taxonomic status. Based on the chloroplast genome structure and composition of this species, it also supports its independent taxonomic status from the Bletilla. Judging from the genetic distance between genera of Orchidaceae, the genetic distance between this sample and any other genera is greater than 0.02, which is greater than the genetic distance between the other two identified genera. In addition, the integration of morphological data from the Flora of China found that there are certain differences in morphology from other species of the genus Bletilla. Therefore, the above results suggest that this group should be a new genus of Orchidaceae.

DOI: 10.1134/S1022795424700030
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An Investigation of Clock Gene Variations in Turkish Nannospalax Species

I. Civelek¹, T. Kankilic¹, D. F. Akin²

¹ Department of Biotechnology, Faculty of Science and Letters, Nigde Omer Halisdemir University, 51000, Nigde, Turkey
² Department of Medical Biology, Faculty of Medicine, Nigde Omer Halisdemir University, 51000, Nigde, Turkey
Correspondence to I. Civelek

Blind mole rats (Nannospalax) have become famous for their long lifespans and cancer resistance, but it is still unknown what causes them to be resistant to cancer. These animals may have a different melatonin synthesis mechanism from other creatures due to their lifestyle, which is estimated to be connected to their cancer resistance. In this study, gene variants in Clock genes Cry1, Cry2, Bmal1, Per1 and Per2, which are involved in the synthesis of melatonin in Nannospalax species living in Turkey were investigated and compared with the other organisms including Nannospalax galili, Mus musculus, Heterocephalus glaber, Rattus norvegicus, and Homo sapiens. In addition, it was investigated whether the detected variations have a pathogenic effect in humans. For this purpose, in vitro methods and some bioinformatics tools were employed in this DNA-based research. 29 variants in total; 11 in Per1, 7 in Per2, 2 in Cry1 and 9 in Cry2 gene, were identified, no variation was found in Bmal1 gene. Some of these variations have been found in regions where DNA repair processes for light-induced UV damage occurred. Other variations were detected in PAS domain and 5'-UTR regions, and the remaining variations were in nondomain regions of proteins. Since Clock gene variations has not been investigated in Nannospalax species, these in vitro and in silico methods may give suggestions to choose and focus on detected variations to be used for further studies.

DOI: 10.1134/S1022795424040057
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Strong Signals of Selection for Body Size in Swine

W. Chen¹, W. Xiao⁵, X. Shi³, J. Li⁴, X. Liao^1,2

¹ College of Life Science, Jinggangshan University, Ji’an, Jiangxi, China
² Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs, Ji’an, Jiangxi, China
³ Ji’an Science and Technology Innovation Center, Ji’an, Jiangxi, China
⁴ Department of Medicine, Jinggangshan University, Ji’an, Jiangxi, China
⁵ Suichuan County Bureau of Agriculture and Rural, Ji’an, Jiangxi, China
Correspondence to X. Liao

Pigs have undergone intense selective pressure throughout their domestication history, and their size is a desirable trait for humans. The objective of this research was to detect the strong selective signals for swine body size using the genome-wide association study (GWAS) approach for ten different pig breeds based on the values of breed genetic differentiation (Fst). Three strong signatures are associated with the size of the pig, with LAGA0046501 at 12.84 Mb on SSC8, ALGA0046498 at 12.51 Mb on SSC8 and a ?600 kb interval from 82.10 to 82.70 Mb on SSC4. LCORL, NCAPG, IMPAD1, PLAG1 have been characterized as plausible candidate genes for body size in swine based on their genomic position and biological function. Our findings provide deeper insights into the genetic architecture of body size in pigs.

DOI: 10.1134/S102279542470008X
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Construction of a Prognostic Risk Prediction Model Based on m6A-Associated Long Non-Coding RNAs in Cholangiocarcinoma

Y. Dai¹, S. Zhang², X. X. Zhang³, J. M. Xu³, Q. He³

¹ Department of General Surgery, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, 441000, Xiangyang, China
² Nephrology Department, Peking University Shougang Hospital, 100043, Beijing, China
³ Department of Hepatobiliary Surgery, Beijing Chao-Yang Hospital Affiliated with Capital Medical University, 10027, Beijing, China
Correspondence to Q. He

To screen long non-coding RNAs (lncRNAs) associated with the N6-methyladenosine (m6A) gene and create a prognostic risk model for cholangiocarcinoma (CCA). Based on the expression levels of lncRNAs and m6A genes, the lncRNAs that related to m6A genes were chosen from the TCGA and EBI databases. Using univariate and multivariate cox regression analysis, lncRNAs associated with prognosis were discovered. A risk score (RS) system was constructed. The correlation between the immune cell infiltration and prognostic risk grouping was further investigated. Finally, the functions of risk-related factors were studied by constructing a ceRNA regulatory network. From the two datasets, 1040 and 410 m6A-associated lncRNAs were identified, respectively. Six m6A-associated lncRNAs (ALDH1L1-AS2, SLC8A1-AS1, FAM27E3, NOP14-AS1, HIF1A-AS2 and F10-AS1) associated with CCA prognosis were obtained. A prognostic risk prediction model was constructed with six m6A-associated lncRNAs. A significant correlation between the low-risk and high-risk groups and actual prognosis. Kaplan–Meier survival curve showed that the higher the RS value of CCA patients, the worse the clinical prognosis. Five significantly different immune cell types were obtained between the low-risk and high-risk groups. Additionally, the immune, ESTIMATE, and stromal scores exhibited significant differences between the two risk groups. Finally, a ceRNA network involving six m6A-associated lncRNAs was constructed. In our study, a prognostic signature consisting of six m6A-associated lncRNAs was constructed with high accuracy for the prediction of prognosis of CCA patients.

DOI: 10.1134/S1022795424700091
К статье на сайте SpringerLink