"Генетика", 2024 г., № 4

Статьи, опубликованные только в Russian J. of Genetics, № 4 – 2024 г.

Expression Profile and Relationships between microRNAs as Biomarkers in COPD Patients

N. Moattar-Husseini¹, N. Bahrami^2,3, F. Hosseini¹, H. Jamaati⁴, M. Kazempour-Dizaji⁵, Sh. Shafaghi⁶, S. Noorali⁶, A. Mohamadnia^4,7,*

¹ Department of Microbiology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, 1956944413, Tehran, Iran
² Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 1956944413, Tehran, Iran
³ Craniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, 1956944413, Tehran, Iran
⁴ Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, 1956944413, Tehran, Iran
⁵ Mycobacteriology Research Center, Biostatistics Unit, NRITLD, Shahid Beheshti University of Medical Sciences, 1956944413, Tehran, Iran
⁶ Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, 1956944413, Tehran, Iran
⁷ Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, 1956944413, Tehran, Iran
Correspondence to A. Mohamadnia

Chronic obstructive pulmonary disease is a chronic inflammatory lung disease that causes airflow obstruction in the lungs. In fact, it is a lung disease that can cause involvement of respiratory tracts, lung tissue or blood vessels. There is still no accurate diagnostic tool for COPD. Among various biomarkers, the current review focuses on different types of miRNAs in COPD which have been studied. Many target cells and molecules, microRNAs are involved in the pathogenesis of COPD. MicroRNAs are a group of protected short single-stranded RNAs between 19 and 23 nucleotides and non-coding, which act as post-transcriptional regulators in animals, plants and viruses. In this article, the aim is to collect and categorize the studies conducted in the field of microRNA as biomarkers in COPD patients.

DOI: 10.1134/S1022795424040082
К статье на сайте SpringerLink

Genome-Wide Identification and Comparative Analysis of Elongation of Very Long-Chain Fatty Acid (Elovl) Genes in Echinoderms

M.-Y. Liu¹, Q.-C. Wang², J.-Q. Li¹, D. Zhang¹, J.-H. Mu¹, X.-H. Shen¹

¹ Yantai Institute of China Agricultural University, 264670, Yantai, PR China
² Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China
Correspondence to X.-H. Shen

Elongation of very long-chain fatty acid (Elovl) proteins is the critical rate-limiting enzyme that catalyzes the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs). Echinoderms are extremely important research objects in various fields, such as regeneration studies and developmental biology. However, to date, the genome-wide characterization and analysis of Elovl genes in echinoderms have not been investigated. In this study, the identification and analysis of Elovl genes in 10 representative echinoderms were performed using bioinformatics methods. A total of 117 Elovl genes have been found in the studied echinoderms. The phylogenetic tree showed that all Elovl genes from echinoderms can be subdivided into 6 classes, Elovl3-like class, Elovl4-like class, Elovl5-like class, Elovl6-like class, Elovl7-like class, and Elovl8-like class. Furthermore, the selection pressure analysis suggested that Elovl genes in echinoderms were mainly constrained by strong purifying selection events. In general, this study provides a molecular basis for echinoderm Elovl genes and may serve as a reference for in-depth phylogenomics.

DOI: 10.1134/S1022795424040070
К статье на сайте SpringerLink

An Exhaustive Structural Scrutiny and Initial Functional Appraisal of the Yak SLC11A1 Gene

L. Wang¹, B. Wang², Z. Gao², Y. Lu², W. Deng², D. Xi², Y. Chong²

¹ Faculty of Biological Engineering, Xinxiang University, 43003, Xinxiang, P. R. of China
² Faculty of Animal Science and Technology, Yunnan Agricultural University, 65201, Kunming, P. R. of China
Correspondence to Y. Chong

Animal diseases pose significant impediments to the flourishing of the animal husbandry sector, due to the role of solute transport protein family 11A1 (SLC11A1) gene on resistance or susceptibility to bacterial infections, rendering it a promising candidate gene for breeding disease-resistant animals. Here, we successfully cloned the coding region of the yak SLC11A1 gene. Comparative analysis against wild yaks and yellow cattle revealed the presence of 3 and 13 base variations, respectively. The N-terminal segment of the yak SLC11A1 gene’s coding region was expressed in a prokaryotic system, the protein exhibited inhibitory activity against Escherichia coli, a pathogen responsible for calf diarrhea. Furthermore, we successfully amplified a gene sequence encompassing a total length of 10 306 bp, and revealing the presence of 15 exons and 14 introns. Within the entire sequence, 14 SNPs were detected, and gave rise to 13 haplotypes. Furthermore, comparative analysis against cattle sequences (KR002419) unveiled two deletion sites, namely g.4511delACCCCACC and g.6410delCAGGT. This comprehensive examination provides insight into the structural attributes of both the coding region and the entire gene sequence, alongside an initial functional evaluation. It lays the foundation for the prospective utilization of the yak SLC11A1 gene as a molecular marker for disease resistance traits.

DOI: 10.1134/S102279542404015X
К статье на сайте SpringerLink